Publications

2024

Structure and stability of an apo thermophilic esterase that hydrolyzes polyhydroxybutyrate.

Thomas GM, Quirk S, Lieberman RL.Acta Crystallogr D Struct Biol. 2024 Nov 1;80(Pt 11):791-799. doi: 10.1107/S2059798324009707. Epub 2024 Oct 23. PMID: 39441250 

Evidence for S₃₃₁-G-S-L within the amyloid core of myocilin olfactomedin domain fibrils based on low-resolution 3D solid-state NMR spectra.

Saccuzzo EG, Robang AS, Gao Y, Chen B, Lieberman RL, Paravastu AK. bioRxiv [Preprint]. 2024 Aug 9:2024.08.09.606901. doi: 10.1101/2024.08.09.606901. PMID: 39149386

SANS reveals lipid-dependent oligomerization of an intramembrane aspartyl protease from H. volcanii.

Thomas GM, Wu Y, Leite W, Pingali SV, Weiss KL, Grant AJ, Diggs MW, Schmidt-Krey I, Gutishvili G, Gumbart JC, Urban VS, Lieberman RL. Biophys J. 2024 Jul 2;123(13):1846-1856. doi: 10.1016/j.bpj.2024.05.029. Epub 2024 Jun 1. PMID: 38824390

2023

Lipid environment modulates processivity and kinetics of a presenilin homolog acting on multiple substrates in vitro

Wu Y, Thomas GM, Thomsen M, Bahri S, Lieberman RL. J Biol Chem. 2023 Dec;299(12):105401. doi: 10.1016/j.jbc.2023.105401.

Molecular basis for inhibition of methane clathrate growth by a deep subsurface bacterial protein.

Huard DJE, Johnson AM, Fan Z, Kenney LG, Xu M, Drori R, Gumbart JC, Dai S, Lieberman RL, Glass JB. PNAS Nexus. 2023 Aug 14;2(8):pgad268. doi: 10.1093/pnasnexus/pgad268. eCollection 2023 Aug. PMID: 37644917 

Myocilin misfolding and glaucoma: A 20-year update.

Saccuzzo EG, Youngblood HA, Lieberman RL. Prog Retin Eye Res. 2023 Jul;95:101188. doi: 10.1016/j.preteyeres.2023.101188. Epub 2023 May 20. PMID: 37217093 

Quantitative differentiation of benign and misfolded glaucoma-causing myocilin variants on the basis of protein thermal stability.

Scelsi HF, Hill KR, Barlow BM, Martin MD, Lieberman RL. Dis Model Mech. 2023 Jan 1;16(1):dmm049816. doi: 10.1242/dmm.049816. Epub 2023 Jan 13. PMID: 36579626

2022

Correlation between chemical denaturation and the unfolding energetics of Acanthamoeba actophorin.

Thota N, Quirk S, Zhuang Y, Stover ER, Lieberman RL, Hernandez R.Biophys J. 2023 Jul 25;122(14):2921-2937. doi: 10.1016/j.bpj.2022.11.2941. Epub 2022 Dec 2. PMID: 36461639

Mapping H+ in the Nanoscale (A2C4)2-Ag8 Fluorophore.

David F, Setzler C, Sorescu A, Lieberman RL, Meilleur F, Petty JT. J Phys Chem Lett. 2022 Dec 8;13(48):11317-11322. doi: 10.1021/acs.jpclett.2c03161. Epub 2022 Dec 1. PMID: 36453924

Change in Morphology of Dimyristoylphosphatidylcholine/Bile Salt Derivative Bicelle Assemblies with Dodecylmaltoside in the Disk and Ribbon Phases.

Leite WC, Wu Y, Pingali SV, Lieberman RL, Urban VS. J Phys Chem Lett. 2022 Oct 27;13(42):9834-9840. doi: 10.1021/acs.jpclett.2c02445. Epub 2022 Oct 17. PMID: 36250687

Bioplastic degradation by a polyhydroxybutyrate depolymerase from a thermophilic soil bacterium.

Thomas GM, Quirk S, Huard DJE, Lieberman RL. Protein Sci. 2022 Nov;31(11):e4470. doi: 10.1002/pro.4470. PMID: 36222314 

Calcium dysregulation potentiates wild-type myocilin misfolding: implications for glaucoma pathogenesis.

Saccuzzo EG, Martin MD, Hill KR, Ma MT, Ku Y, Lieberman RL.J Biol Inorg Chem. 2022 Sep;27(6):553-564. doi: 10.1007/s00775-022-01946-3. Epub 2022 Jul 13. PMID: 35831671

Structure and activity of a thermally stable mutant of Acanthamoeba actophorin.

Quirk S, Lieberman RL. Acta Crystallogr F Struct Biol Commun. 2022 Apr 1;78(Pt 4):150-160. doi: 10.1107/S2053230X22002448. Epub 2022 Mar 28. PMID: 35400667

Consensus Recommendation for Mouse Models of Ocular Hypertension to Study Aqueous Humor Outflow and Its Mechanisms.

McDowell CM, Kizhatil K, Elliott MH, Overby DR, van Batenburg-Sherwood J, Millar JC, Kuehn MH, Zode G, Acott TS, Anderson MG, Bhattacharya SK, Bertrand JA, Borras T, Bovenkamp DE, Cheng L, Danias J, De Ieso ML, Du Y, Faralli JA, Fuchshofer R, Ganapathy PS, Gong H, Herberg S, Hernandez H, Humphries P, John SWM, Kaufman PL, Keller KE, Kelley MJ, Kelly RA, Krizaj D, Kumar A, Leonard BC, Lieberman RL, Liton P, Liu Y, Liu KC, Lopez NN, Mao W, Mavlyutov T, McDonnell F, McLellan GJ, Mzyk P, Nartey A, Pasquale LR, Patel GC, Pattabiraman PP, Peters DM, Raghunathan V, Rao PV, Rayana N, Raychaudhuri U, Reina-Torres E, Ren R, Rhee D, Chowdhury UR, Samples JR, Samples EG, Sharif N, Schuman JS, Sheffield VC, Stevenson CH, Soundararajan A, Subramanian P, Sugali CK, Sun Y, Toris CB, Torrejon KY, Vahabikashi A, Vranka JA, Wang T, Willoughby CE, Xin C, Yun H, Zhang HF, Fautsch MP, Tamm ER, Clark AF, Ethier CR, Stamer WD.Invest Ophthalmol Vis Sci. 2022 Feb 1;63(2):12. doi: 10.1167/iovs.63.2.12. PMID: 35129590 

Catalytically active holo Homo sapiens adenosine deaminase I adopts a closed conformation.

Ma MT, Jennings MR, Blazeck J, Lieberman RL. Acta Crystallogr D Struct Biol. 2022 Jan 1;78(Pt 1):91-103. doi: 10.1107/S2059798321011785. Epub 2022 Jan 1. PMID: 34981765

2021 

Improved resolution crystal structure of Acanthamoeba actophorin reveals structural plasticity not induced by microgravity.

Quirk S, Lieberman RL. Acta Crystallogr F Struct Biol Commun. 2021 Dec 1;77(Pt 12):452-458. doi: 10.1107/S2053230X21011419. Epub 2021 Nov 11. PMID: 34866600

E. coli Ribonucleotide Reductase β2 Subunit Inactivation by Triapine Occurs through Binding of a Triapine-Fe(II) Adduct.

Safiarian MS, Watson RA, Lieberman RL, Barry BA, Offenbacher AR. J Phys Chem Lett. 2021 Sep 23;12(37):9020-9025. doi: 10.1021/acs.jpclett.1c02103. Epub 2021 Sep 13. PMID: 34516127

Molecular architecture and modifications of full-length myocilin.

Martin MD, Huard DJE, Guerrero-Ferreira RC, Desai IM, Barlow BM, Lieberman RL. Exp Eye Res. 2021: 108729. doi: https://doi.org/10.1016/j.exer.2021.108729. PMID: 34400147

Recombinant antibodies recognize conformation-dependent epitopes of the leucine zipper of misfolding-prone myocilin.

Patterson-Orazem AC, Qerqez AN, Azouz LR, Ma MT, Hill SE, Ku Y, Schildmeyer LA, Maynard JA, Lieberman RL.J Biol Chem. 2021 Sep;297(3):101067. doi: 10.1016/j.jbc.2021.101067. Epub 2021 Aug 9. PMID: 34384785 

Common and rare myocilin variants: Predicting glaucoma pathogenicity based on genetics, clinical, and laboratory misfolding data.

Scelsi HF, Barlow BM, Saccuzzo EG, Lieberman RL. Hum Mutat. 2021 Aug;42(8):903-946. doi: 10.1002/humu.24238. Epub 2021 Jun 24. PMID: 34082484 

Optically Modulated and Optically Activated Delayed Fluorescent Proteins through Dark State Engineering.

Peng B, Dikdan R, Hill SE, Patterson-Orazem AC, Lieberman RL, Fahrni CJ, Dickson RM. J Phys Chem B. 2021 May 27;125(20):5200-5209. doi: 10.1021/acs.jpcb.1c00649. Epub 2021 May 12. PMID: 33978414

Preparation of a Deuterated Membrane Protein for Small-Angle Neutron Scattering.

Wu Y, Weiss KL, Lieberman RL. Methods Mol Biol. 2021;2302:219-235. doi: 10.1007/978-1-0716-1394-8_12. PMID: 33877630

Molecular Insights into Myocilin and Its Glaucoma-Causing Misfolded Olfactomedin Domain Variants.

Lieberman RL, Ma MT. Acc Chem Res. 2021 May 4;54(9):2205-2215. doi: 10.1021/acs.accounts.1c00060. Epub 2021 Apr 13. PMID: 33847483

Structural Arrangement within a Peptide Fibril Derived from the Glaucoma-Associated Myocilin Olfactomedin Domain.

Gao Y, Saccuzzo EG, Hill SE, Huard DJE, Robang AS, Lieberman RL, Paravastu AK. J Phys Chem B. 2021 Mar 25;125(11):2886-2897. doi: 10.1021/acs.jpcb.0c11460. Epub 2021 Mar 8. PMID: 33683890

Archaeal roots of intramembrane aspartyl protease siblings signal peptide peptidase and presenilin.

Raut P, Glass JB, Lieberman RL. Proteins. 2021 Feb;89(2):232-241. doi: 10.1002/prot.26009. Epub 2020 Oct 1. PMID: 32935885

2020

Mainly on the Plane: Deep Subsurface Bacterial Proteins Bind and Alter Clathrate Structure.

Johnson AM, Huard DJE, Kim J, Raut P, Dai S, Lieberman RL, Glass JB. Cryst Growth Des. 2020 Oct 7;20(10):6290-6295. doi: 10.1021/acs.cgd.0c00855. Epub 2020 Jul 23. PMID: 33414686 

A blueprint for academic laboratories to produce SARS-CoV-2 quantitative RT-PCR test kits.

Mascuch SJ, Fakhretaha-Aval S, Bowman JC, Ma MTH, Thomas G, Bommarius B, Ito C, Zhao L, Newnam GP, Matange KR, Thapa HR, Barlow B, Donegan RK, Nguyen NA, Saccuzzo EG, Obianyor CT, Karunakaran SC, Pollet P, Rothschild-Mancinelli B, Mestre-Fos S, Guth-Metzler R, Bryksin AV, Petrov AS, Hazell M, Ibberson CB, Penev PI, Mannino RG, Lam WA, Garcia AJ, Kubanek J, Agarwal V, Hud NV, Glass JB, Williams LD, Lieberman RL. J Biol Chem. 2020 Nov 13;295(46):15438-15453. doi: 10.1074/jbc.RA120.015434. Epub 2020 Sep 3. PMID: 32883809 

Substrate-Enzyme Interactions in Intramembrane Proteolysis: γ-Secretase as the Prototype.

Liu X, Zhao J, Zhang Y, Ubarretxena-Belandia I, Forth S, Lieberman RL, Wang C. Front Mol Neurosci. 2020 May 19;13:65. doi: 10.3389/fnmol.2020.00065. eCollection 2020. PMID: 32508589 

2019

Different Grp94 components interact transiently with the myocilin olfactomedin domain in vitro to enhance or retard its amyloid aggregation.

Huard DJE, Jonke AP, Torres MP, Lieberman RL. Sci Rep. 2019 Sep 4;9(1):12769. doi: 10.1038/s41598-019-48751-8. PMID: 31484937

Calcium-ligand variants of the myocilin olfactomedin propeller selected from invertebrate phyla reveal cross-talk with N-terminal blade and surface helices.

Hill SE, Cho H, Raut P, Lieberman RL. Acta Crystallogr D Struct Biol. 2019 Sep 1;75(Pt 9):817-824. doi: 10.1107/S205979831901074X. Epub 2019 Aug 22. PMID: 31478904 

Progranulin deficiency leads to reduced glucocerebrosidase activity.

Zhou X, Paushter DH, Pagan MD, Kim D, Nunez Santos M, Lieberman RL, Overkleeft HS, Sun Y, Smolka MB, Hu F. PLoS One. 2019 Jul 10;14(7):e0212382. doi: 10.1371/journal.pone.0212382. eCollection 2019. PMID: 31291241 

Stable calcium-free myocilin olfactomedin domain variants reveal challenges in differentiating between benign and glaucoma-causing mutations.

Hill SE, Kwon MS, Martin MD, Suntharalingam A, Hazel A, Dickey CA, Gumbart JC, Lieberman RL. J Biol Chem. 2019 Aug 23;294(34):12717-12728. doi: 10.1074/jbc.RA119.009419. Epub 2019 Jul 2. PMID: 31270212 

Antibodies Used to Detect Glaucoma-Associated Myocilin: More or Less Than Meets the Eye?

Patterson-Orazem AC, Lieberman RL. Invest Ophthalmol Vis Sci. 2019 May 1;60(6):2034-2037. doi: 10.1167/iovs.19-26843. PMID: 31067323 

How does a protein’s structure spell the difference between health and disease? Our journey to understand glaucoma-associated myocilin.

Lieberman RL. PLoS Biol. 2019 Apr 22;17(4):e3000237. doi: 10.1371/journal.pbio.3000237. eCollection 2019 Apr. PMID: 31009450 

Exploring substituent diversity on pyrrolidine-aryltriazole iminosugars: Structural basis of β-glucocerebrosidase inhibition.

Martínez-Bailén M, Carmona AT, Patterson-Orazem AC, Lieberman RL, Ide D, Kubo M, Kato A, Robina I, Moreno-Vargas AJ. Bioorg Chem. 2019 May;86:652-664. doi: 10.1016/j.bioorg.2019.02.025. Epub 2019 Feb 12. PMID: 30825709

Differential Misfolding Properties of Glaucoma-Associated Olfactomedin Domains from Humans and Mice.

Patterson-Orazem AC, Hill SE, Wang Y, Dominic IM, Hall CK, Lieberman RL. Biochemistry. 2019 Apr 2;58(13):1718-1727. doi: 10.1021/acs.biochem.8b01309. Epub 2019 Mar 12. PMID: 30802039 

Atomic Structure of a Fluorescent Ag8 Cluster Templated by a Multistranded DNA Scaffold.

Huard DJE, Demissie A, Kim D, Lewis D, Dickson RM, Petty JT, Lieberman RL. J Am Chem Soc. 2019 Jul 24;141(29):11465-11470. doi: 10.1021/jacs.8b12203. Epub 2019 Jan 2. PMID: 30562465 

2018

Neutron scattering in the biological sciences: progress and prospects.

Ashkar R, Bilheux HZ, Bordallo H, Briber R, Callaway DJE, Cheng X, Chu XQ, Curtis JE, Dadmun M, Fenimore P, Fushman D, Gabel F, Gupta K, Herberle F, Heinrich F, Hong L, Katsaras J, Kelman Z, Kharlampieva E, Kneller GR, Kovalevsky A, Krueger S, Langan P, Lieberman R, Liu Y, Losche M, Lyman E, Mao Y, Marino J, Mattos C, Meilleur F, Moody P, Nickels JD, O’Dell WB, O’Neill H, Perez-Salas U, Peters J, Petridis L, Sokolov AP, Stanley C, Wagner N, Weinrich M, Weiss K, Wymore T, Zhang Y, Smith JC. Acta Crystallogr D Struct Biol. 2018 Dec 1;74(Pt 12):1129-1168. doi: 10.1107/S2059798318017503. Epub 2018 Dec 20. PMID: 30605130

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling.

Oliver RC, Naing SH, Weiss KL, Pingali SV, Lieberman RL, Urban VS. J Vis Exp. 2018 Oct 21;(140):57901. doi: 10.3791/57901. PMID: 30394373 

Progress toward development of a proteostasis drug for myocilin-associated glaucoma.

Huard DJ, Lieberman RL. Future Med Chem. 2018 Jun 1;10(12):1391-1393. doi: 10.4155/fmc-2018-0073. Epub 2018 May 23. PMID: 29788763 

Epitope mapping of commercial antibodies that detect myocilin.

Patterson-Orazem AC, Hill SE, Fautsch MP, Lieberman RL. Exp Eye Res. 2018 Aug;173:109-112. doi: 10.1016/j.exer.2018.05.002. Epub 2018 May 9. PMID: 29752947 

Simulations and Experiments Delineate Amyloid Fibrilization by Peptides Derived from Glaucoma-Associated Myocilin.

Wang Y, Gao Y, Hill SE, Huard DJE, Tomlin MO, Lieberman RL, Paravastu AK, Hall CK. J Phys Chem B. 2018 Jun 7;122(22):5845-5850. doi: 10.1021/acs.jpcb.8b03000. Epub 2018 May 21. PMID: 29724098 

Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability.

Goldenzweig A, Goldsmith M, Hill SE, Gertman O, Laurino P, Ashani Y, Dym O, Unger T, Albeck S, Prilusky J, Lieberman RL, Aharoni A, Silman I, Sussman JL, Tawfik DS, Fleishman SJ. Mol Cell. 2018 Apr 19;70(2):380. doi: 10.1016/j.molcel.2018.03.035. PMID: 29677494

Consensus recommendations for trabecular meshwork cell isolation, characterization and culture.

Keller KE, Bhattacharya SK, Borrás T, Brunner TM, Chansangpetch S, Clark AF, Dismuke WM, Du Y, Elliott MH, Ethier CR, Faralli JA, Freddo TF, Fuchshofer R, Giovingo M, Gong H, Gonzalez P, Huang A, Johnstone MA, Kaufman PL, Kelley MJ, Knepper PA, Kopczynski CC, Kuchtey JG, Kuchtey RW, Kuehn MH, Lieberman RL, Lin SC, Liton P, Liu Y, Lütjen-Drecoll E, Mao W, Masis-Solano M, McDonnell F, McDowell CM, Overby DR, Pattabiraman PP, Raghunathan VK, Rao PV, Rhee DJ, Chowdhury UR, Russell P, Samples JR, Schwartz D, Stubbs EB, Tamm ER, Tan JC, Toris CB, Torrejon KY, Vranka JA, Wirtz MK, Yorio T, Zhang J, Zode GS, Fautsch MP, Peters DM, Acott TS, Stamer WD.Exp Eye Res. 2018 Jun;171:164-173. doi: 10.1016/j.exer.2018.03.001. Epub 2018 Mar 9.PMID: 29526795 

Solution Structure of an Intramembrane Aspartyl Protease via Small Angle Neutron Scattering.

Naing SH, Oliver RC, Weiss KL, Urban VS, Lieberman RL. Biophys J. 2018 Feb 6;114(3):602-608. doi: 10.1016/j.bpj.2017.12.017. PMID: 29414706 

Trifunctional High-Throughput Screen Identifies Promising Scaffold To Inhibit Grp94 and Treat Myocilin-Associated Glaucoma.

Huard DJE, Crowley VM, Du Y, Cordova RA, Sun Z, Tomlin MO, Dickey CA, Koren J 3rd, Blair L, Fu H, Blagg BSJ, Lieberman RL. ACS Chem Biol. 2018 Apr 20;13(4):933-941. doi: 10.1021/acschembio.7b01083. Epub 2018 Feb 20. PMID: 29402077

Both positional and chemical variables control in vitro proteolytic cleavage of a presenilin ortholog.

Naing SH, Kalyoncu S, Smalley DM, Kim H, Tao X, George JB, Jonke AP, Oliver RC, Urban VS, Torres MP, Lieberman RL. J Biol Chem. 2018 Mar 30;293(13):4653-4663. doi: 10.1074/jbc.RA117.001436. Epub 2018 Jan 30. PMID: 29382721 

2017

Isoform-selective Hsp90 inhibition rescues model of hereditary open-angle glaucoma.

Stothert AR, Suntharalingam A, Tang X, Crowley VM, Mishra SJ, Webster JM, Nordhues BA, Huard DJE, Passaglia CL, Lieberman RL, Blagg BSJ, Blair LJ, Koren J 3rd, Dickey CA. Sci Rep. 2017 Dec 20;7(1):17951. doi: 10.1038/s41598-017-18344-4. PMID: 29263415 

Structure and Misfolding of the Flexible Tripartite Coiled-Coil Domain of Glaucoma-Associated Myocilin.

Hill SE, Nguyen E, Donegan RK, Patterson-Orazem AC, Hazel A, Gumbart JC, Lieberman RL. Structure. 2017 Nov 7;25(11):1697-1707.e5. doi: 10.1016/j.str.2017.09.008. PMID: 29056483 

Myocilin Regulates Metalloprotease 2 Activity Through Interaction With TIMP3.

Joe MK, Lieberman RL, Nakaya N, Tomarev SI. Invest Ophthalmol Vis Sci. 2017 Oct 1;58(12):5308-5318. doi: 10.1167/iovs.16-20336. PMID: 29049729 

De novo design of antibody complementarity determining regions binding a FLAG tetra-peptide.

Entzminger KC, Hyun JM, Pantazes RJ, Patterson-Orazem AC, Qerqez AN, Frye ZP, Hughes RA, Ellington AD, Lieberman RL, Maranas CD, Maynard JA. Sci Rep. 2017 Aug 31;7(1):10295. doi: 10.1038/s41598-017-10737-9. PMID: 28860479 

Second Generation Grp94-Selective Inhibitors Provide Opportunities for the Inhibition of Metastatic Cancer.

Crowley VM, Huard DJE, Lieberman RL, Blagg BSJ. Chemistry. 2017 Nov 7;23(62):15775-15782. doi: 10.1002/chem.201703398. Epub 2017 Sep 27. PMID: 28857290 

Metal ion coordination in the E. coli Nudix hydrolase dihydroneopterin triphosphate pyrophosphatase: New clues into catalytic mechanism.

Hill SE, Nguyen E, Ukachukwu CU, Freeman DM, Quirk S, Lieberman RL. PLoS One. 2017 Jul 25;12(7):e0180241. doi: 10.1371/journal.pone.0180241. eCollection 2017. PMID: 28742822 

2016

Enzymatic hydrolysis by transition-metal-dependent nucleophilic aromatic substitution.

Kalyoncu S, Heaner DP Jr, Kurt Z, Bethel CM, Ukachukwu CU, Chakravarthy S, Spain JC, Lieberman RL. Nat Chem Biol. 2016 Dec;12(12):1031-1036. doi: 10.1038/nchembio.2191. Epub 2016 Oct 3. PMID: 27694799 

Lessons from an α-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization.

Johnson JL, Kalyoncu S, Lieberman RL. Methods Mol Biol. 2016;1432:281-301. doi: 10.1007/978-1-4939-3637-3_18. PMID: 27485343

Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability.

Goldenzweig A, Goldsmith M, Hill SE, Gertman O, Laurino P, Ashani Y, Dym O, Unger T, Albeck S, Prilusky J, Lieberman RL, Aharoni A, Silman I, Sussman JL, Tawfik DS, Fleishman SJ. Mol Cell. 2016 Jul 21;63(2):337-346. doi: 10.1016/j.molcel.2016.06.012. Epub 2016 Jul 14. PMID: 27425410 

Development of Glucose Regulated Protein 94-Selective Inhibitors Based on the BnIm and Radamide Scaffold.

Crowley VM, Khandelwal A, Mishra S, Stothert AR, Huard DJ, Zhao J, Muth A, Duerfeldt AS, Kizziah JL, Lieberman RL, Dickey CA, Blagg BS. J Med Chem. 2016 Apr 14;59(7):3471-88. doi: 10.1021/acs.jmedchem.6b00085. Epub 2016 Apr 4. PMID: 27003516 

Discovery of Molecular Therapeutics for Glaucoma: Challenges, Successes, and Promising Directions.

Donegan RK, Lieberman RL. J Med Chem. 2016 Feb 11;59(3):788-809. doi: 10.1021/acs.jmedchem.5b00828. Epub 2015 Sep 25. PMID: 26356532 

2015

Increased Fab thermoresistance via VH-targeted directed evolution.

Entzminger KC, Johnson JL, Hyun J, Lieberman RL, Maynard JA. Protein Eng Des Sel. 2015 Oct;28(10):365-77. doi: 10.1093/protein/gzv037. Epub 2015 Aug 16. PMID: 26283664 

Molecular Details of Olfactomedin Domains Provide Pathway to Structure-Function Studies.

Hill SE, Donegan RK, Nguyen E, Desai TM, Lieberman RL. PLoS One. 2015 Jun 29;10(6):e0130888. doi: 10.1371/journal.pone.0130888. eCollection 2015. PMID: 26121352 

Catalytic Properties of Intramembrane Aspartyl Protease Substrate Hydrolysis Evaluated Using a FRET Peptide Cleavage Assay.

Naing SH, Vukoti KM, Drury JE, Johnson JL, Kalyoncu S, Hill SE, Torres MP, Lieberman RL. ACS Chem Biol. 2015 Sep 18;10(9):2166-74. doi: 10.1021/acschembio.5b00305. Epub 2015 Jul 15. PMID: 26118406

Structural and biophysical characterization of an epitope-specific engineered Fab fragment and complexation with membrane proteins: implications for co-crystallization.

Johnson JL, Entzminger KC, Hyun J, Kalyoncu S, Heaner DP Jr, Morales IA, Sheppard A, Gumbart JC, Maynard JA, Lieberman RL. Acta Crystallogr D Biol Crystallogr. 2015 Apr;71(Pt 4):896-906. doi: 10.1107/S1399004715001856. Epub 2015 Mar 27. PMID: 25849400 

Structural basis for misfolding in myocilin-associated glaucoma.

Donegan RK, Hill SE, Freeman DM, Nguyen E, Orwig SD, Turnage KC, Lieberman RL. Hum Mol Genet. 2015 Apr 15;24(8):2111-24. doi: 10.1093/hmg/ddu730. Epub 2014 Dec 18. PMID: 25524706 

2014

Exploiting the interaction between Grp94 and aggregated myocilin to treat glaucoma.

Stothert AR, Suntharalingam A, Huard DJ, Fontaine SN, Crowley VM, Mishra S, Blagg BS, Lieberman RL, Dickey CA. Hum Mol Genet. 2014 Dec 15;23(24):6470-80. doi: 10.1093/hmg/ddu367. Epub 2014 Jul 15. PMID: 25027323

Molecular basis of 1-deoxygalactonojirimycin arylthiourea binding to human α-galactosidase a: pharmacological chaperoning efficacy on Fabry disease mutants.

Yu Y, Mena-Barragán T, Higaki K, Johnson JL, Drury JE, Lieberman RL, Nakasone N, Ninomiya H, Tsukimura T, Sakuraba H, Suzuki Y, Nanba E, Mellet CO, García Fernández JM, Ohno K. ACS Chem Biol. 2014 Jul 18;9(7):1460-9. doi: 10.1021/cb500143h. Epub 2014 May 12. PMID: 24783948

Effects of protein engineering and rational mutagenesis on crystal lattice of single chain antibody fragments.

Kalyoncu S, Hyun J, Pai JC, Johnson JL, Entzminger K, Jain A, Heaner DP Jr, Morales IA, Truskett TM, Maynard JA, Lieberman RL. Proteins. 2014 Sep;82(9):1884-95. doi: 10.1002/prot.24542. Epub 2014 Mar 24. PMID: 24615866 

The glaucoma-associated olfactomedin domain of myocilin forms polymorphic fibrils that are constrained by partial unfolding and peptide sequence.

Hill SE, Donegan RK, Lieberman RL. J Mol Biol. 2014 Feb 20;426(4):921-35. doi: 10.1016/j.jmb.2013.12.002. Epub 2013 Dec 9. PMID: 24333014 

Ligands for glaucoma-associated myocilin discovered by a generic binding assay.

Orwig SD, Chi PV, Du Y, Hill SE, Cavitt MA, Suntharalingam A, Turnage KC, Dickey CA, France S, Fu H, Lieberman RL. ACS Chem Biol. 2014 Feb 21;9(2):517-25. doi: 10.1021/cb4007776. Epub 2013 Dec 9. PMID: 24279319 

2013

Determination of soluble and membrane protein structures by X-ray crystallography.

Lieberman RL, Peek ME, Watkins JD. Methods Mol Biol. 2013;955:475-93. doi: 10.1007/978-1-62703-176-9_25. PMID: 23132076

2012

New direction for glaucoma therapeutics: focus on the olfactomedin domain of myocilin.

Donegan RK, Lieberman RL. Future Med Chem. 2012 Nov;4(17):2131-4. doi: 10.4155/fmc.12.132. PMID: 23190098 

The glaucoma-associated olfactomedin domain of myocilin is a novel calcium binding protein.

Donegan RK, Hill SE, Turnage KC, Orwig SD, Lieberman RL. J Biol Chem. 2012 Dec 21;287(52):43370-7. doi: 10.1074/jbc.M112.408906. Epub 2012 Nov 5. PMID: 23129764 

Glucose-regulated protein 94 triage of mutant myocilin through endoplasmic reticulum-associated degradation subverts a more efficient autophagic clearance mechanism.

Suntharalingam A, Abisambra JF, O’Leary JC 3rd, Koren J 3rd, Zhang B, Joe MK, Blair LJ, Hill SE, Jinwal UK, Cockman M, Duerfeldt AS, Tomarev S, Blagg BS, Lieberman RL, Dickey CA. J Biol Chem. 2012 Nov 23;287(48):40661-9. doi: 10.1074/jbc.M112.384800. Epub 2012 Oct 3. PMID: 23035116 

2011

Amyloid fibril formation by the glaucoma-associated olfactomedin domain of myocilin.

Orwig SD, Perry CW, Kim LY, Turnage KC, Zhang R, Vollrath D, Schmidt-Krey I, Lieberman RL. J Mol Biol. 2012 Aug 10;421(2-3):242-55. doi: 10.1016/j.jmb.2011.12.016. Epub 2011 Dec 13. PMID: 22197377 

A Guided Tour of the Structural Biology of Gaucher Disease: Acid-β-Glucosidase and Saposin C.

Lieberman RL. Enzyme Res. 2011;2011:973231. doi: 10.4061/2011/973231. Epub 2011 Nov 22. PMID: 22145077 

Binding of 3,4,5,6-tetrahydroxyazepanes to the acid-β-glucosidase active site: implications for pharmacological chaperone design for Gaucher disease.

Orwig SD, Tan YL, Grimster NP, Yu Z, Powers ET, Kelly JW, Lieberman RL. Biochemistry. 2011 Dec 13;50(49):10647-57. doi: 10.1021/bi201619z. Epub 2011 Nov 14. PMID: 22047104 

Sneak peak at galactocerebrosidase, Krabbe disease’s lysosomal hydrolase.

Lieberman RL. Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15017-8. doi: 10.1073/pnas.1112653108. Epub 2011 Sep 6. PMID: 21896758 

Crystallization chaperone strategies for membrane proteins.

Lieberman RL, Culver JA, Entzminger KC, Pai JC, Maynard JA. Methods. 2011 Dec;55(4):293-302. doi: 10.1016/j.ymeth.2011.08.004. Epub 2011 Aug 11. PMID: 21854852 

The stability of myocilin olfactomedin domain variants provides new insight into glaucoma as a protein misfolding disorder.

Burns JN, Turnage KC, Walker CA, Lieberman RL. Biochemistry. 2011 Jul 5;50(26):5824-33. doi: 10.1021/bi200231x. Epub 2011 Jun 9. PMID: 21612213 

Biophysical characterization of the olfactomedin domain of myocilin, an extracellular matrix protein implicated in inherited forms of glaucoma.

Orwig SD, Lieberman RL. PLoS One. 2011 Jan 24;6(1):e16347. doi: 10.1371/journal.pone.0016347. PMID: 21283635 

Conversion of scFv peptide-binding specificity for crystal chaperone development.

Pai JC, Culver JA, Drury JE, Motani RS, Lieberman RL, Maynard JA. Protein Eng Des Sel. 2011 May;24(5):419-28. doi: 10.1093/protein/gzq120. Epub 2011 Jan 8.  PMID: 21217145 

2010

Rescue of glaucoma-causing mutant myocilin thermal stability by chemical chaperones.

Burns JN, Orwig SD, Harris JL, Watkins JD, Vollrath D, Lieberman RL. ACS Chem Biol. 2010 May 21;5(5):477-87. doi: 10.1021/cb900282e. PMID: 20334347